To better understand the relationship between gut barrier function and the symptomatology and
pathophysiology of IBS.
- Age 18 to 70 years
- No restrictions on Hospital Anxiety and Depression score
- No abdominal surgery (except appendectomy).
- Written informed consent
- Females who are pregnant, breastfeeding or hysterectomized.
- Use of tobacco products within the past 6 months (since nicotine may affect intestinal
- Use of NSAIDs or aspirin within the past week (since NSAIDs affect intestinal
- Use of oral corticosteroids within the previous 6 weeks
- Ingestion of artificial sweeteners such as Splenda TM (sucralose), Nutrasweet TM
(aspartame), lactulose or mannitol 2 days before the study tests begins, e.g. foods to
be avoided are sugarless gyms or mints and diet soda.
- Ingestion of any prescription, over the counter, or herbal medications which can
affect gastrointestinal transit 7 days before the study begins.
- Proton pump inhibitors.
- Antibiotics for the preceding 60 days before the start of the study.
- (vii) Alcohol intake beyond the recommended safe limit (<21 unites per week)
- Bleeding disorders or medications that increase risk of bleeding from mucosal
- Known allergy to fluorescein
- Inflammatory bowel diseases, celiac disease.
- Clinically significant bradycardia with potential to interfere with procedure induced
vagal tone (Investigators may be guided by a cut-off heart rate of < 50