To evaluate the use of a point of care device to measure markers of inflammation in various
eye conditions. In particular, matrix metalloproteinase-9 (MMP-9) will be measured. MMP-9 is
an enzyme that plays a role in inflammation. The value obtained with the point of care device
will be correlated with values obtained using gel electrophoresis to measure MMP-9 from the
same sample. These data will be compared to clinical exam findings and questionnaires to help
the investigators better understand the role of this marker of inflammation in eye diseases
and possibly improve diagnostic abilities.
As a participant in the study a sample of tears will be collected in a non-invasive manner by
sitting comfortably at the slit lamp (a slit lamp is the standard device used to examine the
eye and merely requires the participant to sit upright and place their chin on a chin rest
and their forehead up against the slit lamp apparatus). The participant will then be asked to
look upwards and while gently lowering the lower eyelid, a sterile blunt glass pipette will
be used to collect tears from the natural tear lake (the tear lake is a reservoir of tears
that sits on the surface of the eye and above the lower eyelid). Collected tears will then be
taken for analysis of inflammatory markers.
In addition, a survey to evaluate the symptoms and the symptoms impact daily life (Ocular
Surface Disability Index Survey of National Eye Institute Visual Functioning Questionnaire-
- Patients that present to clinic for evaluation. Patients with and without evidence of
eye inflammation will be eligible for this study.
- Patients outside the age limits will not be eligible for the study