Collect exhaled breath condensates from patients scheduled for a routine surgical procedure
before, during, and after surgery for measurements of IsoFs
Draw blood from patients scheduled for a routine surgical procedure before, during, and
after surgery for measurements of serum thrombomodulin.
High concentrations of oxygen are routinely administered to patients undergoing routine
surgical procedures even though it is highly likely, at least in patients with normal lung
function, that this is unnecessary and in fact may be doing unnecessary harm. Therefore, we
plan to assess whether there is any evidence of lung injury during surgery resulted from
administration of high concentrations of oxygen by measuring Isofurans (IsoFs) in exhaled
breath condensate. In our studies in mice that were exposed to high concentrations of oxygen
we also found that circulating levels of thrombomodulin were increased. Thrombomodulin is
present in endothelial cells and upon injury they release thrombomodulin into the
circulation. Endothelial cell injury is known to occur in hyperoxic-induced lung injury.
Therefore, in addition to measuring IsoFs in exhaled breath condensate we will also measure
plasma concentrations of thrombomodulin as an indicator of endothelial injury.
- American Society of Anesthesiologists (ASA) Class I-III.
- Patient is undergoing elective surgery requiring mechanical ventilation
- Patient is an adult, 18 - 80 years old.
- Subject has voluntarily signed and dated the informed consent document approved by
the Institutional Review Board (IRB).
- Surgical procedures boarded for less than 2 hours
- Current Smoker
- All Cardiac, Thoracic and/or Vascular surgeries involving the chest
- Patient unable to cooperate.
- Chronic airway diseases
- Medical contraindication to anesthetic technique (allergy, cardiac condition,
neurologic condition, localized infection, bleeding disorder).
- Subjects who are pregnant.