Monocentric study to assess the safety and immunogenicity of the recombinant MVA-HIV
multiantigen vaccine MVA-mBN120B in HIV-infected subjects. 15 subjects will receive
immunizations at day 0, after 4 and 12 weeks at a dose of 2E8 TCID50 MVA-mBN120B. The
vaccine will be administered subcutaneously.
- Male and female subjects aged between 18 to 55 years.
- HIV-1 infection documented by either plasma HIV-1 RT-PCR or bDNA assay at any time
prior to study entry.
- Stable on HAART with regard to immunologic and clinical parameters for at least 6
consecutive months prior to study entry (substitutions of one drug for another in the
same class due to reasons other than virologic failure are allowed).
- Plasma HIV RNA level < 50 copies/ml for at least 6 months prior to study entry;
single blips of up to 400 HIV-1 RNA copies/ml are acceptable if they resolve
spontaneously without a change in HAART.
- Plasma HIV-1 RNA levels of < 50 copies/ml at study entry.
- Women with childbearing potential must have a negative serum pregnancy test at
screening and a negative urine pregnancy test within 24 hours prior to immunization.
- Women of childbearing potential must have used an acceptable method of contraception
for 30 days prior to the first immunization, must agree to use an acceptable method
of contraception during the study, and must not become pregnant for at least 28 days
after the last immunization. A woman is considered of childbearing potential unless
post-menopausal or surgically sterilized. (Acceptable contraception methods are
restricted to abstinence, barrier contraceptives, intrauterine contraceptive devices
or licensed hormonal products.)
- CD4 cells ≥ 350/µl (two determinations, of which one must be done during the
screening and the other must be done within 3 months before study entry)
- CD4 nadir > 150/µl.
- Troponin I within normal institutional limits.
- White blood cells ≥ 2500/mm3 and < = 11,000/ mm3.
- Negative urine glucose by dipstick or urinalysis at screening.
- Haemoglobin ≥ 9.0g/dL.
- Platelets ≥ 100,000/mm3
- Adequate renal function defined as:
1. Calculated Creatinine clearance (CrCl) > 50 mL/min as estimated by the
2. Urine protein < = 100 mg/dL or none or trace proteinuria (by urinalysis or dip
stick (< = 2+ proteinuria)).
- Adequate hepatic function defined as:
1. Total bilirubin < = 2 x ULN unless elevation is explained by antiviral therapy
and in the absence of other evidence of significant liver disease (healthy
subjects without clinical disease with Gilbert's Syndrome can be included).
2. AST (SGOT), ALT (SGPT) and alkaline phosphatase < = 3 x ULN without clinically
- Electrocardiogram (ECG) without abnormal findings (e.g. any kind of atrioventricular
or intraventricular conditions or blocks such as complete left or right bundle branch
block, AV-node block, QTc or PR prolongation, premature atrial contractions or other
atrial arrhythmia, sustained ventricular arrhythmia, two premature ventricular
contractions (PVC) in a row, ST elevation consistent with ischemia).
- Negative test for hepatitis B surface Antigen (HBsAg) and hepatitis C antibody (HCV
- Free of obvious health problems as established by medical history and physical
examination before entering into the study.
- Signed informed consent form of the subject after information of the risks and
benefits of the study in a language the subject clearly understands, and before any
study specific procedure.
- Availability for follow-up for the planned duration of the study.
- Pregnant or breast-feeding women.
- Uncontrolled serious infection i.e. not responding to antimicrobial therapy.
- History of any serious medical condition, which in the opinion of the investigator
would compromise the safety of the subject.
- History of suspected or active autoimmune disease. Persons with vitiligo or thyroid
disease taking thyroid replacement therapy are not excluded.
- History of chronic alcohol abuse (40g / day for at least 6 months) and/or intravenous
drug abuse (within the past 6 months).
- History of malignancy, other than squamous cell or basal cell skin cancer, unless
there has been surgical excision that is considered to have achieved cure. Subjects
with history of skin cancer at the vaccination site are excluded.
- Manifestation of clinically significant and severe haematological, pulmonary, central
nervous, cardiovascular or gastrointestinal disorder.
- Clinically significant mental disorder not adequately controlled by medical
- Any condition which might interfere with study objectives or would limit the
subject's ability to complete the study or to be compliant in the opinion of the
- History of coronary heart disease, myocardial infarction, angina, congestive heart
failure, cardiomyopathy, stroke or transient ischemic attack, uncontrolled high blood
pressure, or any other heart condition under the care of a doctor.
- History of an immediate family member (father, mother, brother, or sister) who died
due to ischemic heart disease before age 50 years.
- Ten percent or greater risk of developing a myocardial infarction or coronary death
within the next 10 years using the National Cholesterol Education Program's risk
assessment tool. (http://hin.nhlbi.nih.gov/atpiii/calculator.asp?usertype=prof)
NOTE: This criterion applies only to volunteers 20 years of age and older.
- History of allergic disease or reactions likely to be exacerbated by any component of
- Known allergy to egg or aminoglycoside (gentamicin).
- History of anaphylaxis or severe allergic reaction.
- Having received any vaccinations or planned vaccinations with a live vaccine within
30 days prior or after study vaccination.
- Having received any vaccinations or planned vaccinations with a killed vaccine
within14 days prior or after study vaccination.
- Chronic administration (defined as more than 14 days) of systemic immuno-suppressant
drugs during a period starting from six months prior to administration of the vaccine
and ending at study conclusion. (Corticosteroid nasal sprays are permissible. Persons
who have used topical and inhaled steroids can be enrolled after their therapy is
- Post organ transplant subjects whether or not receiving chronic immunosuppressive
- Any continuous therapy that may influence CD4 counts other than antiretroviral
- Administration or planned administration of immunoglobulins and/or any blood products
during a period starting from 3 months prior to administration of the vaccine and
ending at study conclusion.
- Use of any investigational or non-registered drug or vaccine other than the study
vaccine within 30 days or 7 half-lives (whichever is longer) preceding the first dose
of the study vaccine, or planned administration of such a drug during the study
- Previous participation in another HIV vaccination study.
- Previous participation in any MVA vaccination study during the last 5 years.