The purpose of the research is to determine which inflammatory substances are involved in
causing allergic symptoms in the eye. Allergic conjunctivitis is a common problem with
symptoms of temporary redness, itching, tearing, and swelling of the eyes. Substances
released by cells in the affected tissues cause allergic reactions in the eye and elsewhere
in the body.
Ocular allergies are extremely common, affecting up to 80 million people in the USA. Our
research question is:
Are there differences in inflammatory mediators and cell surface activation markers in
patients undergoing seasonal allergic conjunctivitis compared to those with sight
threatening disease such as Atopic Keratoconjunctivitis (AKC) and will the use of the
anti-allergy eye drop, PATANOL® (olopatadine hydrochloride) affect these parameters?
Ocular surface cells (by impression cytology) and tears (via capillary tube) are collected
from allergic, non-allergic, and AKC subjects undergoing an reaction induced either by
seasonal allergen or topical allergen provocation (specificity and dose determined via skin
testing). Ocular surface cells are evaluated for surface activation markers. Tears are
evaluated for mediator content. Tears are also incubated with peripheral blood eosinophils
and lymphocytes to see effects on adhesion to conjunctival epithelial cells.
- Skin test positive
- Able to put drops in eyes
- Able to have tears collected