The purpose of this proposal is clinical validation of an electrochemical biochip for rapid
pathogen identification and antibiotic susceptibility determination.
Point-of-care identification of pathogens and determination of antibiotic susceptibility
will significantly improve the clinical management of urinary tract infection. We have
previously developed a biochip based on microfabrication technology capable of rapid
detection of pathogens. The specific objectives of the current proposal are: 1)
Determination of microbial constituents in spinal cord injury (SCI) patients and development
of additional species-specific probes against these pathogens; 2) Development of a rapid
antibiotic susceptibility and molecular pyuria assay using the electrochemical biochip; and
3) Clinical validation of the biochip as a diagnostic test for urinary tract infection.
Within a single protocol, two non-interventional studies were conducted at different time
points to achieve the aforementioned objectives. Sensitivity and specificity of the
electrochemical biosensor based assay was demonstrated in each study.
- Patients suspected or at risk for complicated urinary tract infections
- Gross contamination of urine samples at the time of collection