The purpose of this research study is to determine the genotype (genetic composition) and
phenotype (physical appearance) of cells derived from the abdominal cavity to determine
whether stem cells are present.
The purpose of this study is to determine the presence and characterization of stem cells in
human peritoneal cavity. Peritoneal lavage fluid from patients who undergo elective open
abdomen will be collected for this purpose. Peritoneal lavage is normally a routine part of
abdominal surgical procedure and usually is discarded after surgery. The patient will
undergo surgical procedure as usual and there will be no added procedures during surgery.
This study will collect and analyze peritoneal fluid for the existence and characteristics
of stem cells from 15 patients undergoing scheduled elective intra-abdominal surgery by
designated surgeons at Cedars-Sinai Medical Center. The types of abdominal surgery could be
open abdominal surgery or laparoscopic surgery. Peritoneal washing is routinely done in
abdominal surgeries. We will collect these fluids to analyze whether stem cells exist in
human peritoneal cavity. The fluids will be collected in a sterile fashion. The collected
fluid will be further processed in the laboratory for the purpose of the study.
Medical record review will be conducted. Information collected will include age, gender,
past and current medical history, current indication for intra-abdominal surgery, substance
exposure history, medication history which will be obtained from review of patients' medical
records. The information will be maintained using coded entry and there will be no link
between patient identifiers to the study codes.
1. 20-60 years old
2. Male or females who are scheduled to undergo elective abdomen surgery
2. Pregnant women
3. Known personal or family history of genetic disorders
4. Known history of malignancy
5. Known history of advanced liver or renal disease
6. HIV Positive: only patients who have tested negative during routine testing of their
donated blood prior to surgery will be included in the study.
7. Hepatitis B and C positive
8. Scheduled to undergo lap banding