The study quantified functional measures of red blood cell mass and oxygen in neonatal
tissues using a non-invasive optical technique: near infrared optical spectroscopy . The
study will determine the absolute concentrations of deoxygenated and oxygenated hemoglobin
and calculate the tissue hemoglobin saturation and total hemoglobin concentration in viv.
Near infrared optical spectroscopy technique use to assessing and defining tissue status in
the anemic state and the tissue's response to transfusions, as well as in monitoring
hemoglobin and hematocrit without pain or loss of blood.
Near infrared optical spectroscopy measurements will be conducted on the muscle, on the
upper leg,arm or on the back, abdomen and the head. The actual location will depend upon the
neonate position and size. A small soft plastic optical fibers probe will be placed to the
select study areas and thhe time of the measurement will not exceed 1 hour.
NIRS measurements of hemoglobin and hemoglobin saturation in tissues before and after
red-cell transfusions in VLBW infants will determine the true effect of the transfusions,
and develop more substantial guidelines regarding indications for transfusion. The accuracy
of conventional (invasive) measurements of Hb and Hct could be comparison with results from
the NIRS technique and clinical usefulness of the NIRS technique could be evaluated by
comparison with trends in conventionally measured values taking fluid status into account.
Near-infrared light does not ionize biological tissue and poses no significant health risk.
Since water absorption is low within this spectral range, local heating of the tissue is
also minimal. Burns and heat damage to the neonate are highly unlikely.
- infants weighing less than 1500 grams at birth
- infants receiving at least one packed red blood cell transfusion
- neonates who are too unstable as determined by the clinicians for additional human