This study will evaluate a test designed to measure immune system responses to HIV and HIV
Assays for HIV-specific human CD4 and CD8 T cell immunity are needed in order to evaluate
the immune response to HIV vaccines. Such assays should be robust, reproducible, and
amenable to high throughput analysis of clinical specimens. Cytokine flow cytometry (CFC)
assays can reliably and specifically detect human CD4 and CD8 T cell responses to
AIDS-related opportunistic infections, including those caused by cytomegalovirus,
Mycobacterium tuberculosis, the Mycobacterium avium complex, cryptococcus, and human
papillomavirus. The purpose of this study is to devise and evaluate a similar CFC assay for
the detection and quantitation of CD4 and CD8 T cell responses against HIV.
This study will evaluate a "Gag-IFNg CFC" assay by comparing the results of this assay with
results from other assays of immune phenotype and function in long-term nonprogressors,
untreated patients with progressive HIV disease, and recipients of candidate HIV vaccines.
The study will also examine HIV-specific immune responses in HIV infected individuals who
appear to exhibit significant immune protection from HIV disease.
Participants in this study will be drawn from other studies currently underway. As a part
of those studies, participants will have regular blood tests. Blood samples from those
studies will be used in this study. No participants will be directly enrolled in this
Blood samples from 5 cohorts of HIV infected individuals and 2 cohorts of HIV uninfected
individuals will be evaluated.
Varying stages of HIV disease are represented in these cohorts, including:
- Individuals who have been exposed but who have not seroconverted
- Individuals who have recently seroconverted
- HAART-treated patients who receive immune modulators such as IL-2 and therapeutic
- HAART-treated patients who undergo structured treatment interruptions
- HAART-treated patients who have durable suppression of viremia
- HAART-treated patients who experience incomplete suppression of viremia
- HAART-treated patients followed with careful drug adherence monitoring
- Long-term nonprogressors
- Untreated progressors