1. Determine the feasibility of generation of autologous Acute Myelogenous Leukemia (AML)
or Chronic Myelogenous Leukemia in myeloid blast crisis (CML/BC) derived dendritic cell
activated lymphocytes (DC/AL) in poor prognosis patients.
2. Determine the toxicity of autologous leukemia derived dendritic cell activated
lymphocytes (DC/AL) in patients with AML or CML/BC.
3. Quantitate circulating immune effector cells in patients after infusion of DC/AL.
4. Record the efficacy of AML or CML/BC derived dendritic cells and activated lymphocytes
in promoting and sustaining remission in patients with AML or CML/BC.
Most patients relapsing with AML either fail to achieve second remission or have only brief
remissions. Patients more than 60 years of age or having histories of antecedent
hematological disorders, prior chemotherapy, or poor risk cytogenetics have generally only
short remissions and as a group have two year survivals of less than 10%. Equally patients
with myeloid blast crisis of CML often fail to achieve remission or have responses of only
brief duration. Laboratory studies have shown that AML leukemic blasts may be induced in
culture to differentiate into dendritic cells which in turn may be used activate autologous
lymphocytes to acquire leukemia specific cytotoxicity. This trial will assess the
feasibility of generation of dendritic cell activated lymphocytes, and toxicity and efficacy
of these activated cells given after reinduction chemotherapy. Before this study begins
some toxicity information will have been generated in a trial of similar cells given to CML
- AML patients either after first relapse or at diagnosis a) with high-risk
cytogenetics such as -7, -5, +8, chromosome 9 or 11 abnormality, or b) WBC > 50,000,
or c) age > 60 years*.
- AML patients are eligible for cell collection if they have > 1000 circulating
blasts/mm at diagnosis.
- CML patients in myeloid blast crisis with > 1000 circulating blasts/mm.
- Creatinine <2, Bilirubin <3.
- Age >18.
- Factors which would prevent the patient from receiving or cooperating with the full
course of therapy or understanding the informed consent procedure.
- Concurrent or expected need for therapy with corticosteroids.
- Positive antibody to human immunodeficiency virus I.
- Acute promyelocytic Leukemia (FAB-M3).
- History of overt cardiac failure, systemic autoimmune disease or expected need for
- Patients >60 will be eligible for study but if found to have good prognosis
cytogenetics (inversion (16) or t(8;21)) will subsequently be withdrawn from
study and treated off protocol without infusion of autologous leukemia derived