This study will compare blood from healthy volunteers and with wound fluid and tissue
samples from patients with acute and chronic wounds enrolled in other NIH studies. Chronic
wounds, such as venous leg ulcers, pressure sores, ischemic ulcers and diabetic foot ulcers,
affect more than 4 million Americans each year and cost about $9 billion to treat. The
nature of these wounds is not well understood and treatments are not always successful, for
unknown reasons. Blood collected from healthy volunteers will be used to prepare a model
for studying various processes involved in wound healing.
Normal healthy volunteers 21 years of age and older who do not smoke and have no medical
problems of the heart, bones, muscles, stomach, lungs, blood, or nervous system, do not have
problems going to the bathroom, and have no infections may be eligible for this study.
Participants will be interviewed briefly for information on their date of birth, gender,
ethnic identity and medical history and will have a brief physical examination, including a
check of height and weight, vital signs and heart and lung sounds. About 14 milliliters (2
tablespoons) of blood will be drawn from the arm.
Chronic wounds are "any interruption on the continuity of the body's tissue that requires a
prolonged time to heal, does not heal, or recurs" (Wysocki, 1996). Venous leg ulcers,
pressure sores, ischemic ulcers, and diabetic foot ulcers are examples of chronic wounds.
These kinds of wounds affect over 4 million Americans each year and cost over $9 billion to
treat. The pathophysiology of these wounds is not well understood and therapies directed at
healing these wounds are not always successful for unknown reasons. To better understand
the pathophysiology of these wounds we propose to collect blood by venipuncture from healthy
volunteers. Blood will be used to prepare blood and plasma derived serum for use in an in
vitro wound healing model and Boyden chamber assays to study cell migration, adhesion,
genetic expression, expression of cell surface receptors, and protein expression to
construct a profile of various healing processes. This baseline data will be used for
studying the effect of acute and chronic wound fluids on cell migration, adhesion, genetic
expression, expression of cell surface receptor and protein expression in an in vitro wound
model (protocols to be submitted for each patient population).
Healthy volunteers, 21 years of age and older.
Male or female.
Volunteers with known neurological, cardiac, endocrine, skeletal, gastrointestinal,
immunological, neoplastic, pulmonary, urologic, hematologic, or infectious disease.
Volunteers taking medications to treat a known diagnosed illness.
Children will not be used because chronic wounds are rarely seen in this population.