Phase I trial to study genetic testing and the effectiveness of irinotecan in treating
patients who have solid tumors and lymphoma. Drugs used in chemotherapy use different ways
to stop cancer cells from dividing so they stop growing or die. Genetic testing for a
specific enzyme may help doctors determine whether side effects from or response to
chemotherapy are related to a person's genetic makeup
I. Classify patients with solid tumors or lymphoma according to UGT1A1 promoter (TATA box)
and coding region (Gly71Arg) mutation, and CYP3A4 promoter (G to A) polymorphisms.
II. Identify UGT1A1 enzyme glucuronidator and irinotecan oxidizer phenotypes in these
patients and determine the correlation between the two metabolic reactions in vivo.
III. Determine the relationship between UGT1A1 genotype (promoter and/or coding region
mutation) and CYP3A4 promoter genotype vs gastrointestinal or bone marrow toxicity, and
pharmacokinetics of irinotecan in these patients.
IV. Determine the pharmacokinetics of irinotecan in these patients.
OUTLINE: Patients are genotyped for UGT1A1 enzyme and classified as "Gilbert's" (7/7),
"heterozygotes" (6/7), and "homozygotes for allele 6" (6/6). The DNA is analyzed for the
UGT1A1 coding region mutation (Gly71Arg) and CYP3A4 promoter polymorphism. Patients are also
examined for glucuronidator ratio of SN-38, the active metabolite of irinotecan, and
classified as "low/slow" (very low or zero SN-38G/SN-38 ratio), "intermediate" (less than
50% normal ratio), or "normal".
Patients receive irinotecan IV over 90 minutes once every 3 weeks. Treatment continues for
at least 2 courses in the absence of disease progression or unacceptable toxicity.
- Histologically proven solid tumor or lymphoma
- Responded to irinotecan OR no existing curative therapy
- No leukemia
- Measurable or evaluable disease
- Performance status - Karnofsky 70-100%
- WBC at least 3500/mm^3
- Absolute neutrophil count at least 1500/mm^3
- Platelet count at least 100,000/mm^3
- Bilirubin normal
- SGOT/SGPT less than 5 times upper limit of normal (unless due to disease)
- Creatinine no greater than 1.5 mg/dL
- Creatinine clearance at least 60 mL/min
- Not pregnant or nursing
- Fertile patients must use effective contraception
- No inflammatory bowel disease requiring therapy
- No chronic diarrhea syndrome or paralytic ileus
- At least 2 weeks since prior colony stimulating factor
- At least 4 weeks since prior biologic therapy
- No concurrent biologic therapy
- See Disease Characteristics
- At least 4 weeks since prior chemotherapy (6 weeks for nitrosoureas or mitomycin)
- No other concurrent chemotherapy
- At least 4 weeks since prior radiotherapy to greater than 25% of bone marrow
- No concurrent palliative radiotherapy
- No prior transplant
- No concurrent substrates of UGT1A1 enzyme
- No concurrent inducers or inhibitors of UGT1A1 enzyme activity