Certain patients enrolled in NIH protocol 94-I-0206 at the Clinical Center may be eligible
to participate in one or more of the following new options:
- Donor/recipient extension phase - Both the recipient (HIV-infected twin) and donor
(non-infected twin) will participate in this extension of the CD4-zeta gene therapy
study. It will evaluate the safety and activity of infusing gene-modified CD4+ cells as
well as the modified CD8+ cells.
- Corticosteroid administration - A corticosteroid, such as prednisone, hydrocortisone or
prednisolone, will be added to the interleukin-2 (IL-2) regimen for preventing or
treating side effects of IL-2 such as fever and other flu-like symptoms.
- Extended follow-up - A more intensive follow-up will be scheduled for patients with
substantial numbers of lymphocytes that harbor the CD4-zeta gene. Every 3 months,
participants will have blood tests and specialized tests of CD4 counts, HIV-1 viral
load and numbers of circulating cells containing the CD4-zeta gene every 3 months> the
frequency of follow-up visits may be reduced as time goes by.
- IL-2 continuation - Participants will continue to receive periodic treatment with IL-2
to see how long the genetically modified cells persist in the bloodstream and to
evaluate the long-term response to IL-2.
- Home treatment with interleukin-2 - Participants may receive future IL-2 treatment
cycles at home. Home treatment involves less frequent data and safety monitoring and no
medical evaluations at the Clinical Center except at the beginning of each cycle.
Open-label, comparative, sequentially randomized treatment with genetically unmodified or
modified ex vivo-expanded T-lymphocytes in patients with HIV infection who possess a
seronegative syngeneic twin. Genetic modification consists of introduction of a gene for
HLA-unrestricted "universal" receptors specific for the gp120 HIV envelope protein.
Treatment is divided into Periods I and II.
An identical twin pair, one of whom is seropositive for HIV, the other twin seronegative,
by standard ELISA, PCR, and Western blot testing.
Patients whose CD4+ count is less than 500/mm(3) at entry must have been on FDA-approved
or expanded-access antiretroviral agent(s) for at least 2 months.
Patients with Kaposi's sarcoma are eligible for this study, but must not have received any
systemic therapy for KS within 4 weeks prior to entry. The diagnosis of KS must have been
confirmed by biopsy.
Anticipated survival greater than 6 months and Karnofsky Performance Status greater than
or equal to 60%.
Males or females 18 years of age or older. Every effort will be made to include both
Free from serious psychological or emotional illness and able to provide written informed
EXCLUSION CRITERIA - RECIPIENT:
Unwillingness to comply with current NIH Clinical Center guidelines concerning appropriate
notification of all current sexual partners of an individual regarding his or her HIV
positive sero-status and the risk of transmission of HIV infection.
Recent history of substance abuse unless evidence is provided of an ongoing therapeutic
intervention (i.e. medical therapy or counseling) to control such abuse.
Pregnancy at entry or unwillingness to practice barrier birth control or abstinence during
EXCLUSION CRITERIA - DONOR:
Untreated or inadequately treated medical condition (e.g., cardiopulmonary disease, acute
infection) which, in the judgement of the Principal Investigator, precludes apheresis.
Serologic positivity for Epstein Barr virus, Cytomegalovirus, Hepatitis B or Hepatitis C
if and only if the recipient twin tests seronegative for the corresponding virus.