Researchers plan to study the fat-rich particles, called lipoproteins, which circulate in
the blood. This study is designed to improve understanding of normal, as well as abnormal,
lipoprotein metabolism and the role it plays in the development of hardening of the arteries
Patients participating in this study will receive injections of lipoproteins or
apolipoproteins (the protein component of lipoproteins) that have been isolated and
purified. These lipoproteins will be labeled with small amounts of radioactive material and
sterilized before they are injected into the patient.
Patients participating in the study will be required to have blood samples taken, and
provide urine samples throughout the course of the study. In addition, patient will be
required to follow a specially formulated diet. Patients will be weighed throughout the
course of the study.
We propose to investigate human in vivo lipoprotein metabolism using radiolabeled
apolipoproteins on plasma lipoproteins. Paired kinetic studies using dual-labeled iodinated
lipoproteins and apolipoproteins are performed in healthy volunteer controls with normal
lipids and subjects with dyslipidemia under controlled metabolic conditions. Studies are
designed to formulate metabolic pathways in patients with undefined genetic disorders of
lipid metabolism as well as in healthy volunteers to provide original insights into normal
and pathologic metabolic pathways. All kinetic data is computer analyzed to provide
quantitative data and facilitate direct comparison of multiple studies.
- INCLUSION CRITERIA:
Subjects with new and undefined dyslipidemia-particularly familial disorders with extreme
Healthy normal volunteers - (18 years old or older) controls to investigate the formation
of HDL for prebeta HDL.
Healthy control volunteers are initially screened for secondary causes of hyperlipidemia
and are excluded if they have any clinically significant laboratory abnormality (i.e.,
liver disease, disease, kidney disease, endocrine disease,), chronic medical problems
(i.e., hypertension, migraines, inflammatory bowel, hepatitis, HIV) or require any chronic
medications (i.e., acne, asthma).
The healthy control volunteers weight must fall within the current guidelines for ideal
Unwilling to follow metabolic diet.
Allergic to iodine.
Unable to sign consent form.
Robert D Shamburek, M.D.
National Heart, Lung, and Blood Institute (NHLBI)
Robert D Shamburek, M.D.
Phone: (301) 496-3460